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抗体药物发现加速器

抗体药物发现业务模块集中于纳米抗体制备,构建噬菌体抗体库、抗体筛选与测序、建库及高通量重组抗体表达纯化

IVD原料定制

真核原核体系高品质抗原分离纯化。

亚单位疫苗开发

全面的基因工程疫苗开发体系,抗独特型抗体疫苗方案,合作开发及技术转让等多种合作模式。

动植物疫病检测试剂开发

提供畜/禽/林/渔疫病检测解决方案,擅长胶体金试纸条ELISA试剂盒的开发

课题项目

针对医学领域提供一站式项目服务,其内容涵盖课题设计、实验求证、结果分析及论文撰写服务。

科研服务

为数以万计的科研工作者提供基础的载体构建、基因检测、蛋白表达纯化、大分子相互作用等科研级别技术服务

特色服务&产品

闻道有先后,术业有专攻,钟鼎特色服务位居业界前列

Southern Blot印记杂交检测服务

传统的胶片曝光与最先进的自动化学发光成像检测系统相结合,确保捕获最佳信噪比的成像时间点

乳酸菌蛋白表达服务

基于Nisin诱导的NICE系统的乳酸菌表达平台,提供多种表达方案,包括热诱导表达、食品级别表达以及分泌型表达。

植物激素检测服务

使用液质联用的方法,精确、高效的检测植物激素含量变化。使用的同位素内标,相对外标法,结果更加客观真实。

超级“瞬蓝”SDS-PAGE染色液

彻底取代传统的考染方法,瞬蓝染色液无毒、无味、绿色环保,5分钟染色完成SDS-PAGE,省时省力。

协助用户发表文献

蛋白表达

* Identification and validation of reference genes for quantitative real-time PCR studies in long yellow daylily, Hemerocallis citrina Borani. Feifan hou.PLoS One. 2017, doi: 10.1371/journal.pone.0174933
* Transcriptional profiling analysis of Spodoptera litura larvae challenged with Vip3Aa toxin and possible involvement of trypsin in the toxin activation.Feifei Song et.al.Sci Rep. 2016; 6: 23861.doi: 10.1038/srep23861
* Activation of PPARγ by a Natural Flavonoid Modulator, Apigenin Ameliorates Obesity-Related Inflammation Via Regulation of Macrophage Polarization.et al.Xiujing Feng et al.EBioMedicine. 2016 Jul; 9: 61–76.doi:10.1016/j.ebiom.2016.06.017
* Molecular characterisation of the vitellogenin gene (AlVg) and its expression after Apolygus lucorum had fed on different hosts. Sun Y et al. Pest Management Science, Volume 72, Issue 9, 22 JAN 2016.
* Hydrogen Sulfide Regulates Krüppel‐Like Factor 5 Transcription Activity via Specificity Protein 1 S‐Sulfhydration at Cys664 to Prevent Myocardial Hypertrophy.Guoliang Meng et al. J Am Heart Assoc. 2016 Sep; 5(9): e004160.doi: 10.1161/JAHA.116.004160
* Identification and Comparative Analysis of CBS Domain-Containing Proteins in Soybean (Glycine max) and the Primary Function of GmCBS21 in Enhanced Tolerance to Low Nitrogen Stress.Qingnan Hao et al.Int J Mol Sci. 2016 May; 17(5): 620.doi: 10.3390/ijms17050620
* Expression and prognostic value of E-cadherin in laryngeal cancer. Xiaoyun Qian et al, Acta Oto-Laryngologica Volume 136, 2016 - Issue 7.
* Preparation and characterization of a stable BHK-21 cell line constitutively expressing the Schmallenberg virus nucleocapsid protein. Yongning Zhang et al. Molecular and Cellular Probes Volume 29, Issue 4, August 2015, 244–253
* Recombinant rat CC16 protein inhibits LPS-induced MMP-9 expression via NF-κB pathway in rat tracheal epithelial cells. Min Pang et al.Exp Biol Med (Maywood). 2015 Oct; 240(10): 1266–1278.doi: 10.1177/1535370215570202
* ROS activates JNK-mediated autophagy to counteract apoptosis in mouse mesenchymal stem cells in vitro.Guanyu Liu et.al.Acta Pharmacol Sin. 2015 Dec; 36(12): 1473–1479. doi: 10.1038/aps.2015.101
* ER Stress Mediates TiAl6V4 Particle-Induced Peri-Implant Osteolysis by Promoting RANKL Expression in Fibroblasts.Zhenheng Wang.et al.PLoS One. 2015; 10(9)doi: 10.1371/journal.pone.0137774.
* Characterizing heat shock protein 90 gene of Apolygus lucorum (Meyer-Dür) and its expression in response to different temperature and pesticide stresses. Yang Sun. et.al.Cell Stress Chaperones. 2014 Sep; 19(5): 725–739.doi: 10.1007/s12192-014-0500-0
* Cloning and prokaryotic expression of GmNAC8 gene isolated from soybean.LIU Xiao-qing et al. Jiangsu Journal of Agricultural Sciences,2014, 04.
* Expression and purification of soluble human APRIL in Ecoli using ELP-SUMO tag. Zhang J. et al.Protein Expr Purif. 2014 Mar;95:177-81. doi: 10.1016/j.pep.2013.12.013. Epub 2014 Jan 10.

分子生物学

* Molecular characterization and detection of a spontaneous mutation conferring imidazolinone resistance in rapeseed and its application in hybrid rapeseed production. Hu, M., Pu, H., Kong, L. et al. Mol Breeding (2015) 35: 46. doi:10.1007/s11032-015-0227-3.
* MoDnm1 Dynamin Mediating Peroxisomal and Mitochondrial Fission in Complex with MoFis1 and MoMdv1 Is Important for Development of Functional Appressorium in Magnaporthe. oryzae. Kaili Zhong el.al. PLoS Pathog. 2016 Aug; 12(8): e1005823.doi: 10.1371/journal.ppat.1005823
* Effect of Removing Superior Spikelets on Grain Filling of Inferior Spikelets in Rice. Cuicui You et al. Front Plant Sci. 2016; 7: 1161. Published online 2016 August 5. doi: 10.3389/fpls.2016.01161
* Identification and validation of reference genes for quantitative real-time PCR studies in long yellow daylily, Hemerocallis citrina Borani. Feifan Hou et al.PLoS One. 2017; 12(3): e0174933. doi: 10.1371/journal.pone.0174933
* KR3和Hs1基因聚合对大豆花叶病毒病抗性的影响 Yu XQ. Zhejiang University, 2016.
* 家蚕c-Myc基因的全长cDNA克隆及促细胞增殖功能初探 Xu Hanfu et al.State Key Laboratory of Silkworm Genome Biology,Southwest University; Science of Sericulture,2015 February.
* Isolation and characterization of a proteinaceous α-amylase inhibitor AAI-CC5 from Streptomyces sp. CC5, and its gene cloning and expression.Sun, Z., Lu, W., Liu, P. et al. Antonie van Leeuwenhoek (2015) 107: 345. doi:10.1007/s10482-014-0333-y.
* 猪带绦虫双歧杆菌表达系统pGEX-TSOL18/B.longum的构建及鉴定 Zhou Bi-ying et al. Department of Parasitology,Zunyi Medical College. Chinese Journal of Parasitology and Parasitic Diseases. 2014 March.
* 家蚕卵黄原蛋白受体BmVgR的体外表达 Luo Juan et al, Scientia Agricultura Sinica, 2014 19.
* Deng Y et al.The role of miR-31-modified adipose tissue-derived stem cells in repairing rat critical-sized calvarial defects.2013 Sep;34(28):6717-28. doi: 10.1016/j.biomaterials.2013.05.042. Epub 2013 Jun 13.
* Deng Y et al. Effects of a miR-31, Runx2, and Satb2 regulatory loop on the osteogenic differentiation of bone mesenchymal stem cells.2013 Aug 15;22(16):2278-86. doi: 10.1089/scd.2012.0686. Epub 2013 Apr 27.

 

免疫学

* Luteoloside Acts as 3C Protease Inhibitor of Enterovirus 71 In Vitro.Zeyu Cao et al.PLoS One. 2016; 11(2): e0148693.doi: 10.1371/journal.pone.0148693
* 禾谷镰刀菌几丁质合成酶的功能研究 Zhang XP. Zhejiang University, 2016. (Southern Blot)
* Recombinant rat CC16 protein inhibits LPS-induced MMP-9 expression via NF-κB pathway in rat tracheal epithelial cells.Min Pang et al.Exp Biol Med (Maywood). 2015 Oct; 240(10): 1266–1278.doi: 10.1177/1535370215570202
* Biotic stress-induced expression of mulberry cystatins and identification of cystatin exhibiting stability to silkworm gut proteinases. Liang, J., Wang, Y., Ding, G. et al. Planta (2015) 242: 1139. doi:10.1007/s00425-015-2345-x.
* 醋酸棉酚对人舌鳞癌Tca8113细胞hMLH1蛋白及mRNA表达的影响 Chen WF et al. Journal of Kunming Medical University, 2014, 01.
* Xin Wang, Ping Zhao, Yi Li, Qiying Yi, Sanyuan Ma, Kang Xie, Huifang Chen, Qingyou Xia*. Modifying the mechanical properties of silk fiber by genetically disrupting the ionic environment for silk formation. Biomacromolecules, 2015.
* 金属离子和离子转运蛋白在蚕丝成纤过程中的功能研究 Wang X. Southwest University,2016.
* 白蜡虫脂酰辅酶A还原酶多克隆抗体的制备 Hu YH et al. Forest Research, 2014, 01.
* 草鱼呼肠孤病毒HZ08株S10编码蛋白多克隆抗体制备及其特性分析 Li YG et al. Journal of Fisheries of China, 2014, 03.
* 异常凝血酶DCP基因克隆表达、纯化及单克隆抗体制备与鉴定 Shen P et al. World Chinese Journal of Digestology, 35.
* 新型Resilin-R5融合蛋白的大肠杆菌表达和纯化 Mao ZZ. Acta Agriculturae Zhejiangensis, 2014, 01.
* 罗非鱼无乳链球菌LrrG蛋白的原核表达及免疫原性分析 Chen Xue et al. Journal of Fisheries of Chinsa, 2014, 05.
* 猪带绦虫TSO45W-4B基因的克隆、表达和抗体制备 Zhou BY et al. Chinese Journal of Parasitology and Parasitic Diseases, 2013, 05.
* 猪带绦虫TSOL18基因的表达、纯化和兔抗血清的制备 Zhou BY et al. Chinese Journal of Zoonoses, 2013, 10.
Simultaneous Detection of Fenitrothion and Chlorpyrifos-Methyl with a Photonic Suspension Array.Xuan Wang et al.PLoS One. 2013; 8(6): e66703. doi: 10.1371/journal.pone.0066703
* 罗非鱼无乳链球菌C5a肽酶的原核表达及其免疫原性研究 Li QY. Shanghai Ocean University, 2013.
* 小鼠肝炎病毒N蛋白单克隆抗体的制备及鉴定 Zhou J et al. Chinese Journal of Cellular and Molecular Immunology, 2012, 08.

 

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