GENERAL INFORMATION
Immunogen:	Recombinant SARS-CoV Nucleoprotein / NP Protein (Catalog#40143-V08B)
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant SARS-CoV Nucleoprotein / NP (Catalog#40143-V08B; NP_828858.1; Met1-Ala422). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Ig Type:	Mouse IgG1
Clone ID	08
Specificity:	SARS-CoV Nucleoprotein / NP Has cross-reactivity in ELISA and WB with SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP Protein (Cat# 40588-V08B).
Formulation:	0.2 μm filtered solution in PBS
Storage:	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. PreservativeFree. Avoid repeated freeze-thaw cycles.
Alternative Names:	NP
APPLICATIONS
Applications:	WB、ELISA, FCM, ICC/IF, IHC-P, IP
	(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
RECOMMENDED CONCENTRATION
WB	WB: 1:1000-1:5000
ELISA	ELISA: 1:5000-1:10000 This antibody can be used at 1:5000-1:10000 with the appropriate secondary reagents to detect SARSCoV Nucleoprotein / NP.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-SARS-NP mouse
monoclonal antibody at 1:1000 dilution.

Lane A: SARS-CoV NP Protein (Cat#40143-V08B) (30ng)
Lane B: SARS-CoV NP Protein (Cat#40143-V08B) (5ng)
Lane C: SARS-CoV-2 (2019-nCoV) NP Protein
(Cat#40588-V08B) (30ng)
Lane D: SARS-CoV-2 (2019-nCoV) NP Protein
(Cat#40588-V08B) (5ng)

Secondary
Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.
Performed under reducing conditions.

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