GENERAL INFORMATION
Immunogen:	Recombinant SARS-CoV Spike S1 Subunit Protein (Catalog#40150-V08B1)
Preparation	Produced in rabbits immunized with purified, recombinant SARS-CoV Spike S1 Subunit Protein (Catalog#40150-V08B1; AAX16192.1; Met1-Arg667). The specific IgG was purified by SARS-CoV Spike S1 Subunit affinity chromatography.
Ig Type:	Rabbit IgG
Specificity:	SARS-CoV Spike S1 Subunit Protein Has cross-reactivity in ELISA and WB with SARS-CoV-2 (2019-nCoV) Spike S1 (Cat# 40591-V08H) SARS-CoV-2 (2019-nCoV) Spike RBD (Cat# 40592-V05H)
Formulation:	0.2 μm filtered solution in PBS
Storage:	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative- Free.
APPLICATIONS
Applications:	WB, ELISA, FCM, ICC/IF, IHC-P, IP
	(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
RECOMMENDED CONCENTRATION
Western Blot	WB: 1:1000-1:5000
ELISA	ELISA: 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti SARS-CoV S1 rabbit polyclonal antibody at 1:2000 dilution.

Lane A: SARS-CoV Spike S1 (Cat# 40150-V08B1) (5ng)
Lane B: SARS-CoV-2 (2019-nCoV) Spike S1 (Cat# 40591-V08H) (30ng)
Lane C: SARS-CoV-2 (2019-nCoV) Spike RBD (Cat# 40592-V05H) (30ng)

Secondary
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.
Performed under reducing conditions.

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