SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag)

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GENERAL INFORMATION
Gene Name Synonym: coronavirus spike; cov spike; ncov RBD; ncov s1; ncov s2; ncov spike; NCP-CoV RBD; NCP-CoV s1; NCP-CoV s2; NCPCoV Spike; novel coronavirus RBD; novel coronavirus s1; novel coronavirus s2; novel coronavirus spike; RBD; S1; S2; Spike RBD
Protein Construction: A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (RBD) (YP_009724390.1) (Arg319-Phe541) was expressed with the Fc region of mouse IgG1 at the C-terminus.
Source: 2019-nCoV
Expression Host: HEK293 Cells
QC Testing
Purity: >95 % as determined by SDS-PAGE.
>95 % as determined by SEC-HPLC.
Bio Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag)(40592-V05H), the EC50 of SARS-CoV2 (2019-nCoV) Spike Protein (RBD, mFc Tag) is 15-50 ng/mL. 2. Loaded Human ACE2 Protein (His tag) (Cat. No. 10108-H08H) on SA Biosensor, can bind SARS-CoV-2/2019-nCoV Spike/RBD Protein (RBD, mFc Tag) (Cat. No. 40592-V05H) with an affinity constant of 0.562 nM as determined by Octet RED System.
Endotoxin: <1.0 EU per μg protein as determined by the LAL method
Predicted N terminal: Arg 319
Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) consists of 457 amino acids and predicts a molecular mass of 51.5 kDa.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements
Usage Guide
Stability & Storage: Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.
Avoid repeated freeze-thaw cycles.
Reconstitution: Detailed reconstitution instructions are sent along with the products.

SDS-PAGE:

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SEC-HPLC:

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Affinity:

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Loaded Human ACE2 Protein (His tag) (Cat. No. 10108-H08H) on SA Biosensor, can bind SARS-CoV-2/2019-nCoV Spike/RBD Protein (RBD, mFc Tag) (Cat. No. 40592- V05H) with an affinity constant of 0.562 nM as determined by Octet RED System.



ELISA -Activity:

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Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind 2019-nCoV Spike Protein (RBD, mFc Tag)(40592-V05H),the EC50 of 2019-nCoV Spike Protein (RBD, mFc Tag) is 15-50ng/mL.



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